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Reproducibility of the MSP-PCR method. 200 cDNA fragments were measured. The cDNA of a S.cerevisiae ligated with two tags and simultaneously amplified by MSP-A (horizontal axis, labeled with FAM) and B (vertical axis, labeled with HEX). Thermal cycling was carried out 30 times. The mixture contains different cDNA fragments derived from different sources as co-existent DNA factors. The cDNA ligated with two tags in a mixture was amplified simultaneously in one reaction tube.
