Table 3.
HLA-DR binding prediction by ProPred analysis for the RD1 ORF protein sequence of Rv8371 to Rv3879 and their experimental validation in a Th1-cell assay
| RD1 sequence | ProPred analysis for HLA-DR binding to |
Positive in the Th1 assay, s% |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DRB | 1.1 | 1.3 | 1.4 | 1.7 | 1.8 | 1.11 | 1.13 | 1.15 | 5.1 | all alleles | ||
| Alleles predicted to bind/alleles included in ProPred | ||||||||||||
| Rv3871 | 2/2 | 7/7 | 9/9 | 2/2 | 6/6 | 9/9 | 11/11 | 3/3 | 2/2 | 51/51 (100s%) | 53 | |
| Rv3872 | 2/2 | 6/7 | 9/9 | 0/2 | 3/6 | 9/9 | 10/11 | 2/3 | 2/2 | 43/51 (84s%) | 33 | |
| Rv3873 | 2/2 | 6/7 | 9/9 | 2/2 | 6/6 | 9/9 | 11/11 | 3/3 | 2/2 | 50/51 (98s%) | 68 | |
| Rv3874 | 2/2 | 7/7 | 9/9 | 0/2 | 5/6 | 9/9 | 10/11 | 0/3 | 2/2 | 44/51 (86s%) | 80 | |
| Rv3875 | 1/2 | 7/7 | 9/9 | 2/2 | 3/6 | 9/9 | 11/11 | 1/3 | 0/2 | 43/51 (84s%) | 48 | |
| Rv3976 | 2/2 | 7/7 | 9/9 | 2/2 | 6/6 | 9/9 | 11/11 | 3/3 | 2/2 | 51/51 (100s%) | 50 | |
| Rv3877 | 2/2 | 7/7 | 9/9 | 2/2 | 6/6 | 9/9 | 11/11 | 3/3 | 2/2 | 51/51 (100s%) | 33 | |
| Rv3978 | 2/2 | 7/7 | 9/9 | 2/2 | 5/6 | 9/9 | 11/11 | 2/3 | 2/2 | 49/51 (100s%) | 40 | |
| Rv3879c | 2/2 | 7/7 | 9/9 | 2/2 | 6/6 | 9/9 | 11/11 | 3/3 | 2/2 | 51/51 (100s%) | ND | |
| MPT83P2a | 1/2 | 0/7 | 0/9 | 0/2 | 1/6 | 0/9 | 1/11 | 0/3 | 0/2 | 3/51 (6s%) | 6 | |
| MPT83P14b | 2/2 | 7/7 | 9/9 | 2/2 | 6/6 | 9/9 | 11/11 | 1/3 | 0/2 | 47/51 (92s%) | 65 | |