Table 3.
Immunohistochemistry results of the small intestine of rats on days 2, 4 and 6 following treatment with MTX alone or MTX plus HES
| Parameter | Control group HES group (n = 19) (n = 19) | MTX group (n = 19) | MTX plus HES group (n = 21) | |
|---|---|---|---|---|
| Day 2, % | ||||
| iNOS antigen-positive cell rate | 57.83 ± 13.6 | 77.50 ± 6.12 | 76.50 ± 16.66a | 66.67 ± 5.16 |
| MPO antigen-positive cell rate | 0.00 ± 0.00 | 0.00 ± 0.00 | 16.33 ± 25.30 | 3.33 ± 5.16 |
| IL-8 antigen-positive cell rate | 48.33 ± 1.63 | 63.33 ± 22.51 | 69.83 ± 12.97a | 57.83 ± 10.34a |
| Ki-67 antigen-positive cell rate | 49.00 ± 0.00 | 83.33 ± 13.66 | 6.33 ± 4.68a | 8.67 ± 9.52a |
| Day 4, % | ||||
| iNOS antigen-positive cell rate | 63.33 ± 12.11 | 68.33 ± 17.22 | 80.00 ± 10.95a | 65.00 ± 5.48b |
| MPO antigen-positive cell rate | 0.00 ± 0.00 | 0.00 ± 0.00 | 1.67 ± 4.08 | 1.67 ± 4.08 |
| IL-8 antigen-positive cell rate | 54.16 ± 12.68 | 23.33 ± 10.33 | 52.50 ± 8.57 | 25.00 ± 23.45ab |
| Ki-67 antigen-positive cell rate | 55.50 ± 20.23 | 83.33 ± 8.16 | 27.17 ± 30.27 | 58.17 ± 16.13 |
| Day 6, % | ||||
| iNOS antigen-positive cell rate | 61.00 ± 11.22 | 55.57 ± 27.03 | 50.86 ± 16.83 | 56.67 ± 20.00 |
| MPO antigen-positive cell rate | 0.00 ± 0.00 | 0.00 ± 0.00 | 7.00 ± 18.52 | 0.00 ± 0.00 |
| IL-8 antigen-positive cell rate | 46.29 ± 7.18 | 45.71 ± 29.36 | 35.00 ± 23.91 | 70.00 ± 12.25a, b |
| Ki-67 antigen-positive cell rate | 50.71 ± 9.14 | 75.57 ± 16.45 | 45.00 ± 7.42 | 76.67 ± 17.32a, b |
Data are arithmetical means ± SD.
a
Statistically significant at p < 0.05 as compared to control group.
a
Statistically significant at p < 0.05 as compared to MTX group.