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. 2017 Jun 30;102(9):3470–3479. doi: 10.1210/jc.2017-00849

Figure 3.

Figure 3.

Effects of miR-29a/c-3p knockdown on the FGF2- and VEGFA-induced phosphorylation (P) of AKT1 and ERK1/2. After a 24-hour treatment with vehicle, NC, and miR-29c-3p(i), followed by a 16-hour serum starvation, HUVECs were treated with ECM-b (control), VEGFA (10 ng/mL) or FGF2 (10 ng/mL) for 10 minutes. Protein samples were subjected to western blotting. (a) Representative blots of P-AKT1, T-AKT1, P-ERK1/2, T-ERK1/2, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Effects of miR-29a/c-3p knockdown on FGF2- and VEGFA-induced relative AKT1 (b) and ERK1/2 (c) phosphorylation (P-AKT1/T-AKT1 and P-ERK1/2/T-ERK1/2, respectively). Data are expressed as median ± SD fold of corresponding control within the same transfection treatment (n = 4). Kruskal–Wallis test was performed. #Differ (P < 0.05) from control group with vehicle treatment; *differ (P < 0.05) among transfection treatment groups.