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. 2017 Jun 30;102(9):3470–3479. doi: 10.1210/jc.2017-00849

Figure 4.

Figure 4.

Knockdown of miR-29a/c-3p inhibits VEGFA- and FGF2-stimulated cell migration (a) but not cell proliferation (b) in NT-HUVECs. (a) After treating with vehicle, NC, or miR-29c-3p(i) for 24 hours, confluent cells were scratched followed by treating cells with ECM-b (control), VEGFA, and FGF2 for 20 hours. The scratch areas were measured at 0 and 20 hours. Data normalized to total scratch area at 0 hour are expressed as median ± SD fold of 0 hour (n = 4). Kruskal–Wallis test was performed. #Differ (P < 0.05) from the corresponding control; *differ (P < 0.05) among treatment groups. (b) After treating with Vehicle, NC, and miR-29c-3p(i) for 24 hours and serum-starved in ECM-b for 8 hours, subconfluent cells were treated with ECM-b (control), ECM, VEGFA (10 and 100 ng/mL), or FGF2 (10 and 100 ng/mL). After 42 hours of treatment, cells were subjected to the methylthiazolyl tetrazolium assay. Data are expressed as median ± SD fold of control (n = 6). Kruskal–Wallis test was performed. #Differ (P < 0.05) from control.