Figure 5.

Knockdown of miR-29a/c-3p does not affect the (a) ECM- and (b) FGF2-strengthened monolayer integrity as well as (c) VEGFA-weakened monolayer integrity in NT-HUVECs. After treating with vehicle, NC, or miR-29c-3p(i), cells were cultured until reaching confluent (∼24 hours). Cells were treated with ECM-b (control), ECM, VEGFA (100 ng/mL), or FGF2 (100 ng/mL). The electrical resistance at 4000 Hz was constantly recorded up to 25 hours. Data are expressed as median ± SD fold of control at corresponding time (n = 4). Kruskal–Wallis test was performed. *Differ (P < 0.05) from control within each corresponding transfection treatment.