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. 2017 Jun 22;102(9):3327–3339. doi: 10.1210/jc.2017-00947

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Figure 4. — The effects of androgens on adipose lipid metabolism in the human preadipocyte SGBS cell line. (a) The effect of androgens (T, light-shaded bars; 5α-DHT, dark-shaded bars) on the messenger RNA expression of ACC1, the main provider of malonyl-CoA for fatty acid synthesis. (b) Effect of T and DHT on de novo lipogenesis, determined by incorporation of ¹⁴C acetate into lipid. (c) Effects of T and DHT on β oxidation, determined by ³H₂0 release from ³H-palmitate. (d) Effects of androgens on free fatty acid uptake. All data are presented as the mean ± standard error of the mean of the three to five experiments. Significance levels: *P < 0.05; **P < 0.01; ***P < 0.001 compared with control (analysis of variance with post hoc Tukey test). mRNA, messenger RNA.