Figure 1.
(A) Artificial recruitment of FCP1 protein to promoter template effectively activates transcription. Transfections of 293 cells were carried out using 100 ng of the indicated reporters together with 500 ng of GAL4–FCP1 or GAL4–FIN13 expression vectors as indicated. Each histogram bar represents the mean of at least three independent transfections made in duplicate. (B) Synergy between hTBP and FCP1 recruited to promoter DNA. The G5–38HIV reporter (100 ng) was transfected into 293 together with the indicated effectors (500 ng). In both panels, data are presented as luciferase activity relative to the sample without effector after normalization.
