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. 2017 Sep 6;12(9):e0183074. doi: 10.1371/journal.pone.0183074

Fig 5. Spheroid cultures preserve CEA expression of their original tumours.

Fig 5

(A) Immunostaining of spheroids and corresponding tumours from two different patients for epithelial cell marker EpCAM (red) and adenocarcinoma marker CEA (green). Nuclei are stained with Hoechst (blue). Spheroids were stained after 10 days of culture. Size bars = 50 μm. (B) Significant difference in percentage of EpCAM-positive cells stained for CEA in tumours and spheroid cultures at day 3 and day 10 (p = 0.040), but Bonferroni corrected post hoc analysis did not reach statistical significance (tumour vs. day 3 p = 0.139, tumour vs. day 10 p = 1.000 and day 3 vs. day 10 p = 0.158).