Table 1. E. coli and mouse liver primers and probes used in this study.
| Organism/ Assay type | Gene Name | Primer/Probe | Sequence |
|---|---|---|---|
| E. coli / genomic | 16S genomic region | Probe | /56-FAM/ AC GTT TGA C/ZEN/C TCG TCT TTC GGT GTG T/3IABKFQ/ |
| Forward | GCC AAG ACC AAC CAA TGA CAG CAA | ||
| Reverse | ACC TGC TCT CTT CGC GTA CTG TTT | ||
| E. coli / gene expression | dnaK | Probe | /56-FAM/ TGC TGA GTT /ZEN/ CTT TGG TAA AGA GCC GCG T/3BHQ_1/ |
| Forward | TGG TGG TCA GAC TCG TAT GCC AAT | ||
| Reverse | ATT GCT ACA GCT TCG TCC GGG TTA | ||
| rpoA | Probe | /5HEX/ TTC GTC GTG /ZEN/ CGG CAA CCA TT/3IABkFQ/ | |
| Forward | TAG AAC AGC GTA CCG ACC TG | ||
| Reverse | CGA AAG CTT CCA GTT GTT CA | ||
| srlR | Probe | /5Cy5/ CCA CAG GCA CAA CCA TTC GCA /3IAbRQSp/ | |
| Forward | ATT GGC GCA ATA CTT TGA CA | ||
| Reverse | GCA TGT TCC AGA ATG ACC AG | ||
| mouse liver / gene expression | gapdh | Probe | /56-FAM/ TGC AAA TGG /ZEN/ CAG CCC TGG TG/3IABkFQ/ |
| Forward | GTG GAG TCA TAC TGG AAC ATG TAG | ||
| Reverse | AAT GGT GAA GGT CGG TGT G | ||
| rpl19 | Probe | /5HEX/ TGA CCG CCA/Zen/T ATG TAT CAC AGC CTG /3IABkFQ/ | |
| Forward | GAT GCG CTT GTT TTT GAA CAC | ||
| Reverse | CCG GCT TCT CAG GAG ATA C | ||
| fn1 | Probe | /5Cy5/ CTG ATC GTT GCA TCT GTT TCT GGA GGT/3IAbRQSp/ | |
| Forward | GTT TCC TCG GTT GTC CTT CT | ||
| Reverse | GAC TGT ACT TGT CTA GGC GAA G |
E. coli primers and probes were designed in-house, mouse assays were selected from those available from IDT. 56-FAM: 5' 6-FAM (Fluorescein), ZEN tm IDT Trademark dark quencher, 3IABKFQ: 3’ Iowa Black Fluorescent Quencher, 3BHQ_1: 3’ Black Hole Quencher, 5HEX: 5’ Hexachlorofluorescein, 5Cy5: 5’ Cyanine5; 3IAbRQSp: 3' Iowa Black® RQ-Sp