Table 2. Validation of lyophilized assays.

Genomic E. coli DNA Assay	High DNA Template	Medium DNA Template	Low DNA Template
Control	15.71 +/- 0.16	23.55 +/- 1.5	29.14 +/- 0.15
Lyophilized	16.43 +/- 0.51	22.87 +/- 0.43	29.87 +/- 0.32
E. coli RNA Assay	dnaK-FAM Ct	rpoA-HEX Ct	srlR-Cy5 Ct
Control	25.79 +/- 0.31	24.4 +/- 0.11	29.32 +/- 0.33
Lyophilized	24.99 +/- 1.52	23.27 +/-0.16	28.53 +/- 0.56
Mouse Liver RNA Assay	gapdh-FAM Ct	rpl19-HEX Ct	fn1-Cy5 Ct
Control	21.11 +/- 0.085	19.35 +/- 0.092	21.57 +/- 0.092
Lyophilized	21.59 +/- 0.41	19.33 +/- 0.33	21.17 +/- 0.085

Lyophilized and non-lyophilized assay comparison of qPCR cycle threshold data for E. coli DNA Assay for (High, Mid, Low) DNA template amount, and RT-qPCR cycle threshold data for E. coli and mouse liver RNA. There are no statistical differences between lyophilized assays and commercial non-lyophilized assays. Data shows Ct values +/- SD.