Table 1.
qRTPCR for detecting NF-κB1 and Bcl-2 expressions in the blank control group, no-load plasmid group, NF-κB1 shRNA group, NF-κB1 overexpression group, Bcl-2 shRNA group, and NF-κB1 overexpression + Bcl-2 shRNA group (mean ± SD)
| Group | Cases | U87
|
SHG44
|
||
|---|---|---|---|---|---|
| NF-κB1 | Bcl-2 | NF-κB1 | Bcl-2 | ||
| Blank control | 3 | 1.314±0.027 | 2.027±0.198 | 3.948±0.028 | 4.676±0.184 |
| No-load plasmid | 3 | 1.145±0.125 | 1.706±0.618 | 2.293±1.156 | 3.893±2.506 |
| NF-κB1 silencing | 3 | 0.485±0.049a | 0.302±0.031b | 0.858±0.091a | 1.881±0.438b |
| NF-κB1 overexpression | 3 | 16.625±1.334a | 4.171±0.097b | 14.023±0.591a | 6.002±1.063b |
| Bcl-2 silencing | 3 | 1.220±0.072a | 0.700±0.072b | 2.322±0.540a | 1.666±0.017b |
| NF-κB1 overexpression + Bcl-2 silencing | 3 | 15.818±0.292 | 1.848±0.342b | 12.190±1.433 | 4.121±0.174b |
| F value | 91.249 | 0.433 | 16.025 | 0.230 | |
| P-value | 0.000 | 0.667 | 0.000 | 0.801 | |
Note: Compared to the blank control group and no-load plasmid group:
a
the expression of NF-κB1 in U87 and SHG44, P<0.05;
b
compared with the blank group and no-load plasmid group, the expression of Bcl-2 in U87 and SHG44, P<0.05.
Abbreviations: NF-κB1, nuclear factor-kappa B1; qRTPCR, quantitative real-time polymerase chain reaction.