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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Mol Cancer Res. 2017 May 8;15(8):1051–1062. doi: 10.1158/1541-7786.MCR-17-0089

Fig. 4. Transcriptional activation of tumor suppressor targets of p53 at baseline and after p53 induction.

Fig. 4

(A) Luciferase reporter assay showing luciferase/renilla activity (fold change/empty vector) in H1299 (p53-null) cells overexpressing p53 with a luciferase reporter vector containing 5’CDKN1A-eP53RE, PTEN-eP53RE, or PTEN-pP53RE (constructs shown). Error bars: mean ± s.e.m.; n = 3 experiments. Significance: two-tailed Student’s t-test. (B) MCF7 cells treated with Nutlin-3 for 24 hours. Error bars: mean ± s.d., repeated twice. Transcripts measured by qRT-PCR. Significance: two-tailed students t-test. (C) MCF10A cells treated with 10 µM Nutlin-3 for 6, 10, and 24 hours. Error bars: mean ± s.d., triplicate measurements. (D) U2OS cells treated with 10 µM Nutlin-3 for 24 hours. Error bars: mean ± s.e.m, n = 4 experiments, triplicate measurements. Significance: one-way ANOVA, Dunnett’s correction. (****p≤.0001,***p≤.001,*p≤.05, n.s. p>.05)