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. Author manuscript; available in PMC: 2017 Sep 6.
Published in final edited form as: Exp Eye Res. 2015 Mar 25;134:80–89. doi: 10.1016/j.exer.2015.03.018

Figure 2. Hyperosmolar stress (HOS) decreases secretion of IL-6 and IL-8 in ocular surface cells.

Figure 2

Secreted IL-8 was measured by ELISA in cell culture supernatants of SV40 HCEC cultures under HOS in the presence of LPS (TLR4 ligand) and LPS with IFN-γ. Increased production of IL-8 in the presence of LPS and/or IFN-γ was not observed under HOS but only at iso-osmolar conditions (A). SV40 HCEC were stimulated with Pseudomonas aeruginosa DNA (TLR9 ligand, 10 μg/ml) under HOS for 24 hrs and supernatants were examined for a panel of 13 inflammatory cytokines; among all interrogated cytokines only IL-6 secretion was significantly decreased under HOS when compared to iso-osmolar conditions (B). Data were analyzed using an unpaired Student's t-test where P ≤ 0.05 was considered to be statistically significant when compared to control (*).