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. Author manuscript; available in PMC: 2017 Sep 6.
Published in final edited form as: Exp Eye Res. 2015 Mar 25;134:80–89. doi: 10.1016/j.exer.2015.03.018

Figure 4. Desiccation modulates the expression of TLR4, TLR5 and TLR9 in human corneal epithelial cells in an organ culture model.

Figure 4

TLR mRNA expression by quantitative RT-PCR showed that TLR4 and TLR5 mRNA were up-regulated by 4.81 and 2.51 fold respectively; whereas TLR9 was down-regulated by 0.86 fold when compared to the control (A). Protein expression by western blotting was determined in epithelial cells harvested from human corneas in organ culture for 24 hrs. At the protein level, TLR4 was up-regulated by 10% (B) and TLR9 was down-regulated by 20.47% (C); TLR5 remained unaltered (data not shown). Data are representative of a minimum of three experiments and were analyzed using a one-way ANOVA where P ≤ 0.05 was considered to be statistically significant when compared to control (*).