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(A) Tetrodotoxin (TTX; 1 μM), an inhibitor of voltage-dependent Na+ channels, did not affect ghrelin induced [Ca2+]c increases. (B) The substitution of choline for Na+ rapidly and reversibly blocked the action of ghrelin. Ghrelin was applied at 200 pM (red bars). The traces are the mean values from 5–20 neurons.
