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. 2017 Sep 6;12(9):e0184324. doi: 10.1371/journal.pone.0184324

Fig 3. Niclosamide triggers ER stress and the autophagic response in U-87 MG cells.

Fig 3

(A) U-87 MG cells were treated with 5 μM niclosamide and collected at the indicated time points. Total cell lysates were resolved on SDS-PAGE and immunoblotted with antibodies specific for CHOP and LC3. (B) U-87 MG cells were treated with 2.5 or 5 μM niclosamide and total protein was isolated from lysed cells after 24 h. Lysates were resolved on SDS-PAGE and immunoblotted with LC3-specific antibody. (C) Representative image of U-87 MG cells incubated with 5 μM niclosamide for 24 h and then stained with MDC (0.05 mM). Fluorescence particles in the cytoplasm indicate autophagic vacuoles.