Fig 1. Flowchart of steps taken for R. solani AG1-IA RNA-seq sample preparation and data analysis.

Hyphae of R. solani, grown on ¼ strength PDA (control) or on soybean unifoliate leaves were harvested and processed for RNA sequencing using the Illumina HiSeq 2000 platform. Data were analyzed using standard analytical pipelines for gene annotation and differential expression analysis. Data were further compared using heatmap analysis and gene ontology terms, and affected pathways examined using Kyoto encyclopedia of genes and genomes (KEGG).