Table 1.
List of primer sequences used for RT-PCR.
| Target gene (product length)a | Outer primers | Inner primers | G enbank No. | |
|---|---|---|---|---|
| GAPDH (367, 313 bp) | Forward | AGCCTCGTCCCGTAGACAAAA | TGAAGGTCGGTGTGAACGAATT | XM_001473623.1 |
| Reverse | TTTTGGCTCCACCCCTTCA | GCTTTCTCCATGGTGGTGAAGA | ||
| vGluT2 (267, 199 bp) | Forward | TCATTGCTGCACTCGTCCACT | TTGCCTCAGGAGAGAAACAACC | NM_080853.3 |
| Reverse | GCGCACCTTCTTGCACAAA | TCTTCCTTTTTCTCCCAGCCG | ||
| VIAAT (560, 468 bp) | Forward | GCTGGTGATGACGTGTATCTT | GTATCTTGTACGTCGTGGTGAG | NM_009508.2 |
| Reverse | CGAAGAAGGGCAACGGATAG | GGTTATCCGTGATGACTTCCTT | ||
| CCR2 (456, 214 bp) | Forward | CTGCTCTTCCTGCTCACATTAC | AGCCAGGACAGTTACCTTTG | NM_009915.2 |
| Reverse | CCTGTGCCTCTTCTTCTCATTC | GCAGATGACCATGACAAGTAGA | ||
aNumbers in parentheses indicate sizes of PCR products obtained from outer and inner primers, respectively.