Table 1.
X-ray Data Collection and Refinement Statistics
| Construct | FGF9-FGFR1c |
|---|---|
| Data Collection | |
| X-ray wavelength | 0.97900 |
| Space group | P212121 |
| Unit Cell Dimensions | |
| a, b, c (Å) | 42.159,86.793,136.857 |
| α, β, γ (°) | 90.00,90.00,90.00 |
| Resolution (Å) | 50–2.50(2.54–2.50)a |
| No. measured reflections | 114188 |
| No. unique reflections | 18063(867) |
| Data redundancy | 6.3(6.1) |
| Data completeness (%) | 99.4(97.6) |
| Rsym (%)b | 8.7(37.8) |
| I/sig | 29.8(4.0) |
|
| |
| Refinement | |
| Resolution (Å) | 29.5–2.50(2.57–2.50) |
| No. unique reflections | 18002(1330) |
| No. Reflections (Rfree)c | 1799(133) |
| Rwork/Rfree | 18.26/22.82(21.82/29.73) |
| No. atoms | |
| Protein | 2764 |
| Ligand/Ion | 15 |
| Solvent | 58 |
| R.m.s. deviations | |
| Bond length (Å) | 0.009 |
| Bond angle (°) | 1.208 |
| Average Bfactors (Å2) | |
| Protein | 52.25 |
| Ligand/Ion | 126.63 |
| Solvent | 44.04 |
| Ramachandran Plot | |
| Outliers (%) | 0.00 |
| Allowed (%) | 4.46 |
| Favored (%) | 95.54 |
| Rotamer outliers (%) | 1.68 |
| No. C-Beta Deviations | 0 |
| All-Atom Clashscore | 5.27 |
|
| |
| PDB ID | 5W59 |
a
Values in parenthesis are for the highest resolution shell.
b
Rsym = Σ|I− < I >|/ΣI, where I is the observed intensity of a reflection, and <I> is the average intensity of all the symmetry related reflections.
c
Number of reflections randomly excluded from the refinement for Rfree calculation.