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Mutations reducing interaction between Mos1 transposase monomers. (A–C) Yeast cells expressing wild-type or one of 12 mutant transposases fused to the lexA protein were spotted on medium containing 0.5 (B) or 5 mM 3-AT (C). Only cells expressing the wild-type fusion can grow on the higher concentration. The position of each mutant is indicated in (A). (D) Map showing the position and identity of each of the 12 amino acid substitutions within Mos1 transposase.
