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. 2017 Sep 6;7:10651. doi: 10.1038/s41598-017-11034-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduced RPE/retina function after sub-retinal administration of lentivirus containing shRNA for Kir7.1. (A) Gel electrophoresis showing that Kir7.1 mRNA expression is reduced after Kir7.1 shRNA injection when compared to the un-injected contralateral eye. Full image of the gel is included as Supplemental Fig. 3A. (B) Expression of Kir7.1/b-actin ratio in shRNA injected and un-injected eye (part A) represented in a bar graph. (C) Representative scotopic traces comparing the shRNA-injected mice 14 d post injection, as well as control and PBS-injected mice. (D) Fluorescent image of live RPE sheet of cells displaying the expression of GFP that is fused with the shRNA Kir7.1. (E) Normalized a-wave and (F) normalized b-wave comparing the shRNA injected eye with the PBS injected eye at 30 cd.s/m² at 0, 2, 4, 7, and 14 d post-injection. (G) Comparison of c-wave for eyes injected with Kir7.1 shRNA and PBS at 0, 2, 4, 7 and 14 d post injection. Dotted line represents the control-normalized response from non-injected eyes.