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. 2017 Sep 6;7:10602. doi: 10.1038/s41598-017-10894-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IL-33 induces Akt/eNOS activation and NO production via ST2/TRAF6. (A,B) After transfection with an ST2- or TRAF6-specific siRNA, HDLECs were treated with IL-33 (20 ng/mL, 30 minutes). Akt and eNOS phosphorylation were detected by Western blotting. (C,D) After transfection with the ST2- or TRAF6-specific siRNA, HDLECs were treated with IL-33 (20 ng/mL, 4 hours) and incubated with DAF-FM DA for 1 hour. Relative intracellular NO levels were determined from the fluorescence intensity of DAF-FM. Three independent experiments were performed in duplicate. **p < 0.01, ***p < 0.001. Full-length blots are presented in Supplementary Figure S4.