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. 2017 Sep 6;7:10602. doi: 10.1038/s41598-017-10894-x

Figure 5.

Figure 5

PI3K/Akt/eNOS-mediated NO production is required for IL-33-induced ILA. (A,B) HDLECs were pre-treated with or without wortmannin (100 nmol/L) or NMA (1 mmol/L) for 30 minutes before stimulation with IL-33 (20 ng/mL). (A) Chemotaxis was quantified after a 4 hour incubation. (B) HDLECs tube formation was quantified after a 16 hour incubation. (C) After IL-33 (10 μg/mL) treatment, ILA was quantified in WT and eNOS KO mice in vivo. Three independent experiments were performed in duplicate. *p < 0.05.