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. 2017 Sep 6;7:10701. doi: 10.1038/s41598-017-10882-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Masson’s trichrome staining. Synthesis and deposition of collagen were evaluated using Masson’s trichrome stained granulation tissues of days 8 and 12. The boxed regions in the whole tissue sections (4x; scale bar 25 µm) are displayed at higher magnification (20x; Scale bar 100 µm) as magnified images. CS+LG hydrogel treated tissues showed enhanced collagen synthesis, deposition, and maturation on days 8 and 12, compared to control and CS hydrogel treatment. Yellow arrows indicate the deposition of collagen bundles. C denotes the collagen fibers in treated tissues.