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. 2017 Sep 6;7:10659. doi: 10.1038/s41598-017-09368-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Weak preTCR signal strength in FVB/n mice underlies inefficient β-selection. (A) Summary bar graph of i.c.TCRβ MFI in DN3 and DN4 thymocytes from C57BL/6 and FVB/n mice. (B) Relative level of pTα^a and pTα^b transcripts expressed by the DN3 thymocytes from C57BL/6 and FVB/n mice. ΔCt corresponds to normalised CT value of reaction according to the reference gene GAPDH. *p < 0.05, ns is for not significant. (C) Representative flow cytometry profiles (left) showing i.c.TCRβ and CD71 on DN3 cells from C57BL/6 and FVB/n mice. Percentages of cells are indicated. Summary bar chart (right) shows percentage of DN3 cells that are i.c.TCRβ CD71⁽⁺⁾. (D) CD5 MFI levels on CD71⁽⁺⁾ β-selected DN3 thymocytes (i.c.TCRβ⁽⁺⁾CD71⁽⁺⁾) from C57BL/6 and FVB/n mice. ****p < 0.0001, ***p < 0.001, **p < 0.01, ns is for not significant.