Figure 3.

Reduced unconventional TCRαβ⁽⁺⁾CD8αα⁽⁺⁾ IELs in pTα^a-only transgenic mice. (A) Summary bar chart of DP cell numbers in C57BL/6, pTα-deficient and pTα^a. pTα^−/−mice. (B) Representative flow cytometry profiles of DN3 and DN4 subsets from thymus of C57BL/6, pTα^−/− and pTα^a. pTα^−/− transgenic mice. (C) Bar chart summarizing the DN3 to DN4 ratio in C57BL/6, pTα-deficient and pTα^a. pTα^−/−mice. (D) Summary bar chart of MFI of CD25 on DN3 thymocytes from each strain of mice. (E) Bar chart showing CD5 MFI on β-selected DN3 cells (i.c.TCRβ⁽⁺⁾CD71⁽⁺⁾) from each strain of mice. (F) Representative flow cytometry plots (from n > 6) of IEL populations (TCRαβ⁽⁺⁾CD8αα⁽⁺⁾ and TCRαβ⁽⁺⁾CD8αβ⁽⁺⁾ IELs) from the small intestine of C57BL/6, pTα-deficient and pTα^a. pTα^−/− mice. Percentages of cells are indicated near each gate. ****p < 0.0001, ***p < 0.001, *p < 0.05, ns is for not significant.