Figure 4.

Strong TCR signalling in DN cells of OT-II TCR transgenic mice promotes increased development of unconventional TCRαβ⁽⁺⁾CD8αα⁽⁺⁾ IELs. (A) Summary bar chart of CD5 MFI on Vα2⁽⁻⁾ and Vα2⁽⁺⁾ DN3 thymocytes from TCR-transgenic OT-II mice. (B) Representative flow cytometry profiles of Vα2⁽⁻⁾ and Vα2⁽⁺⁾ DN3 and DN4 subsets from the thymus of TCR-transgenic OT-II mice. (C) Representative flow cytometry plots for TCRαβ⁽⁺⁾ IEL populations (TCRαβ⁽⁺⁾CD8αα⁽⁺⁾ and TCRαβ⁽⁺⁾CD8αβ⁽⁺⁾ IELs) from the small intestine of C57BL/6 and OT-II mice. Right is summary bar chart. Percentages of gated cells are indicated. ****p < 0.0001.