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. 2017 Sep 6;7:10718. doi: 10.1038/s41598-017-11417-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GE induced apoptosis via caspase signaling pathway. After 24 h of GE treatment, the apoptosis-associated protein in HEY and A2780 cells were examined by western blot (A), and caspase-3 activity in HEY cells was tested (B). After 1 h of ZVF and 24 h of GE treatment, the cells were examined by annexin V/PI staining assay (C), and the proteins were examined by western blot (D). The full-length gels of western blot were shown in the Supplementary Information file. All experiments were performed at least three times.