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. 2017 Sep 6;7:10718. doi: 10.1038/s41598-017-11417-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibitory effects of GE on the cell migration and invasion of HEY cells. (A) The movement of HEY cells was observed by wound-healing assay at 0, 24, and 48 h after GE treatment. The migration and invasion abilities of HEY cells were examined by Transwell assay (B, C). Migration-associated proteins (D) and invasion-associated proteins (E) were evaluated by western blot. The activities of extracellular MMPs were determined by gelatin zymography assay (F). The full-length gels were shown in the Supplementary Information file. All experiments were performed at least three times.