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. 2017 Sep 6;7:10657. doi: 10.1038/s41598-017-10728-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Purity analysis, structure confirmation and effect on cell viability of compound Amt-87. (A) The chemical structure of Amt-87 showing atom numbering. (B) HPLC-DAD analysis of Amt-87 performed at 254 nm (up) and 280 nm (down) is presented. The purity of Amt-87 is estimated to be above 98%. (C) The ¹H-NMR (left) and ¹³C-NMR (right) spectra of Amt-87 are shown. The peaks in the spectra are identified and assigned to the structure. (D) The impact of Amt-87 on viability of J-Lat A2 and HeLa cells was measured at the indicated time points after the commencement of treatment by using the Promega CellTiter-Glo® Luminescent Cell Viability Assay kit. The error bars represent mean ± SD from three independent experiments.