Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 6;7:10657. doi: 10.1038/s41598-017-10728-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Amt-87 promotes reversal of HIV latency in a time- and does-dependent manner. (A,B) J-LatA2 cells were treated with 50 μg/ml of Amt-87 for the indicated time periods (A) or the indicated concentrations of the drug for 24 hr (B). The percentages of cells expressing GFP were measured by FACS and plotted. (C) J-LatA2 cells were treated with DMSO or 200 μg/ml Amt-87 for 24 hr. The GFP mRNA levels were measured by qRT-PCR and normalized to those of GAPDH and plotted, with the value in DMSO-treated cells adjusted to 1. (D) Jurkat 2D10 cells were treated with 100 μg/ml of Amt-87 or DMSO for 24 hr and the expression of GFP was analyzed by FACS and plotted. (E,F) The HeLa-based NH1 cells containing an integrated HIV LTR-luciferase reporter gene were treated with 100 μg/ml of Amt-87 for the indicated time periods (E) or 24 hr with the indicated concentrations of the drug (F). (G,H) The NH2 cells expressing Tat were treated with 100 μg/ml of Amt-87 for the indicated time periods (G) or 24 hr with in indicated concentrations of the drug (H). Whole cell extracts were prepared and examined for the luciferase activities. The error bars in all panels represent mean ± SD based on at least three independent experiments.