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. 2017 Sep 6;7:10672. doi: 10.1038/s41598-017-09733-w

Figure 4.

Figure 4

Effects of Ang II on the mRNA and protein expression of molecules related to cholesterol metabolism in podocytes. (A) Podocytes were treated with Ang II (10−7 M) for 24 h. The histogram shows the changes in the relative mRNA levels of the cholesterol metabolism-related molecules ABCA1, SREBP1, SREBP2, HMGCR and LDLR in the two groups, demonstrating that Ang II decreased the mRNA level of ABCA1 but significantly increased the mRNA levels of SREBP1, SREBP2, HMGCR and LDLR. n = 3, *p < 0.05 vs. the normal group. (B) Podocytes were treated with various concentrations of Ang II (10−9–10−5 M) for 24 h. Representative Western blots of ABCA1, SREBP1, SREBP2, HMGCR and LDLR, demonstrating that Ang II decreased the protein level of ABCA 1 but clearly increased the protein levels of SREBP1, SREBP2, HMGCR and LDLR. n = 3, *p < 0.05 vs. the normal group. For clarity lanes were cropped from different gels. Full-length blots are presented in Fig. S4.