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. 2017 Sep 6;7:10656. doi: 10.1038/s41598-017-11152-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) SERS responses in the DA-1 chamber of microfluidic immunoassay during detection of IL-18 in human blood plasma sample according to the subsequent steps of parallel approach: (a) after the mixture of equal amounts of three kinds of Raman reporter-labeled Au nanoparticles was delivered and incubated (5 min) onto the anti-IL18 modified Ag-Au SERS-active surface; (b–f) after subsequent washing by PBS buffer solution. (B) Typical SERS responses for increasing concentration of target IL-6, IL-8, and IL-18 interleukins in blood plasma during a simultaneous multiplexed approach.