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. 2017 Sep 6;7:10633. doi: 10.1038/s41598-017-10410-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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20β-Dihydrocortisol (20β-DHF) is an abundant cortisol metabolite which is increased in plasma and urine of obese horses. (A) Obese horses excreted significantly more urinary β-cortol, β-cortolone and 20β-DHF than lean horses as measured by GC-MS/MS. (B) Plasma 20β-DHF concentrations were significantly higher in obese horses compared to lean horses. (C) Hepatic 20β-DHF concentrations did not differ between lean and obese horses. (D) Visceral adipose 20β-DHF concentrations did not differ between lean and obese horses. Data are mean ± SEM, n = 14/group, *P < 0/05.