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. 2017 Sep 6;7:10739. doi: 10.1038/s41598-017-11208-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of CD137L, CDC42BPG and FST as p53 direct target genes. (a–c) Luciferase assay of the p53BS in human (left) or mouse (right) CD137L (a), CDC42BPG (b) or FST (c) using SaOS2 cells. Luciferase activity is indicated relative to the activity of the mock vector with SD (n = 3). *P < 0.05, *P < 0.001, Student’s t-test. (d–f) CHIP assay for CD137L (d), CDC42BPG (BS-C) (e) or FST (f) was performed using SaOS2 cells that were infected with Ad-LacZ (lane 1) or Ad-p53 (lane 2-4). DNA-protein complexes were immunoprecipitated with an anti-p53 antibody (lanes 1 and 2) followed by qPCR. Input chromatin represents a small portion (1%) of the sonicated chromatin collected prior to immunoprecipitation. Immunoprecipitates with an anti-IgG antibody (lane 3) or in the absence of an antibody (lane 4) were used as negative controls. Columns, mean; error bars, SD (n = 3).