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. 2017 Sep 6;7:10739. doi: 10.1038/s41598-017-11208-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of CD137L as a bone-specific p53 target gene. (a) Induction of Cd137l among 24 tissues. Samples were categorized into 4 groups: (K) p53 ^−/− mice without irradiation, (W) p53 ^+/+ mice without irradiation, (KX) p53 ^−/− mice with irradiation and (WX) p53 ^+/+ mice with irradiation (n = 3 per group). We calculated the median FPKM value of WX/maximum FPKM value of the median FPKM value in K, KX or W for 24 tissues. (b) At 24 h after transfection with each siRNA, U2OS cells were treated with ADR (2 μg/ml for 2 h). At 36 h after treatment, whole cell extracts were subjected to western blotting with an anti-CD137L, anti-p21, anti-p53, or anti-β-actin antibody. siRNA against EGFP was used as a control. β-actin was used for normalization of the expression levels. These images were cropped from full-length blots (Supplementary Fig. 9). (c) Immunohistochemical staining of Cd137l in mouse p53^α or p53^−/− calvaria with or without radiation exposure. Representative images from three tissues in each group are shown. Scale Bars (left) = 50 μm, Scale Bars (right) = 20 μm. Black arrowhead shows osteoblast.