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. 2017 Sep 6;7:10618. doi: 10.1038/s41598-017-10997-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Viability of biofilm in presence of 16~256 µg/mL of methyl gallate. The confocal microscopic images of LIVE/DEAD stained biofilm in (A) drug free control; biofilm treated with (B) furanone 10 µg/mL; (C) 16 µg/mL, (D) 32 µg/mL, (E) 64 µg/mL, (F) 128 µg/mL, and (G) 256 µg/mL of methyl gallate. The viability of the biofilms was assessed using BacLight LIVE/DEAD stain (green: live cells, red: dead cells). In each image, the segment at below right side shows three dimensional and other three segments shows two dimensional images.