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. 2017 Sep 6;8:448. doi: 10.1038/s41467-017-00525-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Midlife Drp1 induction delays age-onset pathology and prolongs healthspan. a Capillary feeding assay (CAFE) of 37 day old daGS > UAS-Drp1 females with or without RU486-mediated transgene induction from day 30 to day 37. n = 8 vials of 10 flies per condition; p > 0.05 and is non-significant (n.s.); two-tailed unpaired t-test. b, c Spontaneous physical activity b of 37 day old daGS > UAS-Drp1 females with or without RU486-mediated transgene induction from day 30 to day 37. Quantification of total activity per fly per hour c from spontaneous activity graphs. n = 3 vials of 10 flies per condition; **p < 0.01; two-tailed unpaired t-test. d Climbing index as a measure of endurance of 37 day old daGS > UAS-Drp1 females with or without RU486-mediated transgene induction from day 30 to day 37. n = 90 flies per condition; ^∗ p < 0.05; two-tailed Mann–Whitney test. e Survival curves without food of 37 day old daGS > UAS-Drp1 females with or without RU486-mediated transgene induction from day 30 to day 37. p < 0.01; log-rank test; n = 100 flies. f Whole body lipid stores of 37 day old daGS > UAS-Drp1 females with or without RU486-mediated transgene induction from day 30 to day 37. n = 5 biological replicates with three flies per replicate; *p < 0.05; two-tailed unpaired t-test. g Fecundity of daGS > UAS-Drp1 females with or without RU486-mediated transgene induction from day 30 to day 37. n = 630 flies on day 10; *p < 0.05; one-way ANOVA/Bonferroni’s multiple comparisons test. h Intestinal integrity during aging of daGS > UAS-Drp1 females with or without RU486-mediated transgene induction since midlife (day 30) onwards. n = 448 flies on day 10; **p < 0.01, *p < 0.05; one-way ANOVA/Bonferroni’s multiple comparisons test. Bars a, c and d depict mean ± s.d. and bars g, h depict mean ± s.e.m. Boxplots f display the first and third quartile, with the horizontal bar at the median and whiskers showing the most extreme data point, which is no more than 1.5 times the interquartile range from the box. RU486 was provided in the media at a concentration of 25 μg/ml