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. 2017 Sep 6;7:10681. doi: 10.1038/s41598-017-11031-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CRMP2 induces GTP-state microtubules through the interaction with soluble GTP-tubulin dimer. (A) Classification of microtubule ends. I, curved sheets; II, blunt ends; III, tapered ends; IV, curved ends. (B) Representative examples for end shapes of GMPCPP-microtubules in the presence of CP525. Red and yellow arrows show class I and class II ends, respectively. (C) Percentage of end types observed. GMPCPP-microtubule (n = 187); GMPCPP-microtubule+CP525 (n = 184); GMPCPP-microtubule+kinesin-1 (nucleotide free) (n = 230); GDP-taxol-microtubule (n = 205). (D) Cryo-EM image of CP525-induced GMPCPP microtubule (top) and its FFT image (bottom) are displayed. (E) Size exclusion chromatography of CP525 (blue), GTP-γS-tubulin-dimer (green), and CP525 with GTP-γS-tubulin-dimer (red) shown with SDS-PAGE results. (F) SEC-MALS analyses of CP525 (top), GTP-γS-tubulin (middle) and their mixture (bottom). (G) The molar mass determined by SEC-MALS experiments. Theoretical molecular weights calculated from the amino acid sequence were also shown for comparison.