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. 2017 Sep 6;7:10671. doi: 10.1038/s41598-017-10979-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Surgical ablation of interscapular BAT alters maternal and fetal metabolic profiles. (A) Schema summarising the key stages of mouse pregnancy over time and the time-points selected for the study of pregnancy and metabolism in iBAT ablated (iBATx) or sham-operated mice, as indicated by the arrows. (B) Maternal weight without uterine horn (Sham, n = 5–10; iBATx, n = 6–9). (C) Maternal serum cholesterol levels (Sham, n = 6–9; iBATx, n = 7–10). (D) Maternal liver triglyceride levels (Sham, n = 7–10; iBATx, n = 9–12). Data are represented as mean ± SEM, *p<0.05 for Sham versus iBATx comparison at a single pregnancy time-point as determined by unpaired Student’s t-test. (E) Weight of fetuses at GD18 (Sham, n = 46 fetuses from 7 mothers; iBATx, n = 51 fetuses from 9 mothers). (F) Weight of placentas at GD18 (Sham, n = 24 placentas from 4 mothers; iBATx, n = 25 placentas from 5 mothers). (G) Average number of fetuses per pregnancy at GD18 (Sham n = 7; iBATx n = 9). *p<0.05 as determined by unpaired Student’s t-test.