Fig. 1.

Identification of cancer-specific essential genes using our gMCS approach. a Example metabolic network. The target reaction is r ₈. b Trivial gene–protein-reaction (GPR) rules scenario for a. Blue coloring is associated with lowly expressed genes, L = {4, 5, 9}, and red coloring is associated with moderately/highly expressed genes or reactions lacking gene annotation, $\overline{L}$={1, 2, 3, 6, 7}. c Resulting gMCSs involving g ₂ given the GPR rules in b. gMCS₂ explains the essentiality of g ₂. d Contingency table with the essentiality predictions of the gMCS approach in the Project Achilles¹⁹ data