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. 2017 Sep 6;7:10658. doi: 10.1038/s41598-017-11263-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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“Characteristic attributes” for RNA2 NNV gene. Abbreviations for column 1: Aus N = North Australia, Aus E = East Australia, Indon = Indonesia, Mal 1a = Kota Kinabalu, Sabah, Malaysia, Mal 1b = Tuaran, Sabah; Malaysia, Mal 1c = Sandakan, Sabah, Malaysia, Mal 1d = UMS hatchery, Sabah, Malaysia, Langkawi; Mal 2 = West Malaysia, Singap = Singapore, Vietna = Vietnam, Taiwan = Taiwan, China S = South China, China C = Central China, China N = North China, Korea = Korea, Japan = Japan.† Different groups that were culled to reduce impact of pseudo-replication, see details in following text. Abbreviations for column 4: F = fish, FW = fish wild, FY = fish wild yearling, C = cell culture. Additional tests for regional differences were conducted using Bayesian Tip-association Significance (BaTS) tests; the association index and the parsimony score statistic assessing regional differences among all samples were both statistically significant (P < 0.05) as were tests of monophyletic clade (MC) sizes for each region, except for Japan and South China.