Fig. 5.

PDGF evokes redoxosome formation. a Serum-starved Swiss 3T3 cells were stimulated with PDGF-BB (50 ng ml⁻¹) for 5 min, then immunostained with anti-PDGFRβ (magenta) and the indicated anti-NOX component antibodies (green). Representative images are shown for each condition from one of two independent experiments. Nuclei were stained with DAPI (blue). Scale bar: 10 μm. The graph shows the average value of Pearson’s coefficient for PDGFRβ and the indicated NOX components with or without PDGF-BB stimulation (n = 1 ROI from each of 10 cells) ***P < 0.0001; two-tailed Welch’s t-test. b Serum-starved Swiss 3T3 cells expressing cytoplasmic HyPer3 (HyPer3-cyto), plasma membrane-targeted HyPer3 (HyPer3-tk), RAB5-fused HyPer3 (HyPer3-RAB5) or RAB7-fused HyPer3 (HyPer3-RAB7) were subjected to live-cell, time-lapse imaging. Top panels: Fluorescence intensity (pseudo-colored) of each HyPer3 construct shown at 1 min intervals. PDGF-BB (50 ng ml⁻¹) was added (arrow) after the third scan. Bottom panel: Graph shows the means of the relative fluorescence intensities of individual cells (n = 7 cells from each independent time-lapse experiment), setting the average of the first three time-points to 1 (bottom). Error bars represent SEM