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. 2017 Sep 6;7:10733. doi: 10.1038/s41598-017-11462-z

Figure 2.

Figure 2

Expression of rhLF in milk. (A) SDS-PAGE analysis of milk from transgenic cloned cows. Milk samples from transgenic cloned cows were collected and detected by SDS-PAGE. hLF, 1 µg of commercial hLF (Sigma) as a positive control; 523, 108, 410, 611, 822, 102, and 811, 0.5 µl of raw milk from transgenic cloned cows; WT, 0.5 µl of raw milk from wild-type cows. (B) Identification of rhLF in milk by western blotting. hLF1, 10 µg of commercial hLF standard (mother solution concentration: 10 µg/µl, undiluted, 1 µl); hLF2, 5 µg of commercial hLF standard (mother solution concentration: 10 µg/µl, dilution 1:2, 1 µl); hLF3, 1 µg of commercial hLF standard (mother solution concentration: 10 µg/µl, dilution 1:10, 1 µl); 523, 108, 102, and 811, 1 µl of raw milk from transgenic cloned cows; 410-1, 611-1, 822-1, 1 µl of milk from transgenic cloned cows; 410-2, 611-2, 822-2, diluted milk from transgenic cloned cows (dilution, 1:10, 1 µl); WT, 1 µl of raw milk from wild-type cows.