Table 1. Kinetic characterization of nucleotide incorporation by human wildtype Pol μ, in the context of a 1-nt gapped DNA substrate.

Primer terminus	Nucleotide	K m (μM)	k cat (s−1)	k cat/Km (s−1·μM−1)
dA	dTTP (4)	4.0 ± 0.90	0.020 ± 0.0050	5.60 × 10−3 ± 2.2 × 10−3
dA	UTP (6)	21.0 ± 3.0	0.04 ± 0.017	2.1 × 10−3 ± 0.65 × 10−3
rA	dTTP (4)	3.7 ± 0.70	0.017 ± 0.0050	4.40 × 10−3 ± 1.0 × 10−3
rA	UTP (3)	23.6 ± 5.80	0.015 ± 0.0007	0.65 ± 0.17 × 10−3

Values represent the mean ± standard deviation for each calculation, with values in parenthesis indicating the number of independent measurements per sample.