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. 2017 May 26;45(15):8773–8784. doi: 10.1093/nar/gkx482

Figure 2.

Figure 2.

The 11.2516 and 11.2521 DHS encompass enhancer elements. (A) The ELF5, EHF and APIP promoters in the pGL3B vector were transfected into 16HBE14o- cells with a Renilla vector as transfection control. Luciferase expression is normalized to Renilla values. n = 3. Open chromatin peaks from 11p13 were inserted into the enhancer sites of the (B) pGL3B-ELF5, (C) pGL3B-EHF and (D) pGL3B-APIP promoter constructs and transfected as in (A). For DHS showing >3-fold luciferase expression relative to the promoter-only construct, n = 3. (E), (F) show transfections into Caco2 cells (n = 2 with each sample assayed in triplicate). (E) The ELF5, EHF, and APIP promoters in the pGL3B vector, (F) the 11.2516 and 11.2521 elements in the enhancer site of pGL3B-EHF. The 11.2516 and 11.2521 elements were cloned in tandem into the (G) pGL3B-ELF5 and (H) pGL3B-EHF promoter constructs and transfected into 16HBE14o- cells, n = 3. For all panels, ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, ns = not significant.