Figure 1.

Experimental outline. (A) Chemical structures of A, m¹A, m⁶A, G, m¹G, m²G and m⁶G. ‘Rib’ indicates ribose. (B) A schematic diagram illustrating the procedures employed for assessing the impact of the methylated ribonucleosides on translation. ‘X’ indicates a methylated adenosine or guanosine. ‘RBS’ and ‘STOP’ designate the ribosome binding site and the stop codon, respectively. Only the mRNA containing an m¹G, m²G, m⁶G or G at the first position of sixth codon of the mRNA, as well as the wild-type peptide product MVGAGGVGK and the competitor peptide product MVVGAGGVGK are shown.