Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
PCR primers used in this study.
| Target gene | Primer sequence (5′ → 3′ direction)* | Amplicon size (bp) | Annealing temperature (°C) | Reference |
|---|---|---|---|---|
| 16 s (SSU) rRNA | 1F: GAGTTTGATCCTGGCTCAG | 1106 | 56 | 36, 37 |
| 12R: AGGGTTGCGCTCGTTG | ||||
| Avicin A | AV-F: ACGCGAAATGAAGAATGTTG | 156 | 55 | 38 |
| AV-R: GACTTCCAACCAGCAGCAC |
*F: forward primer; R: reverse primer.
