Table 3.

Effect of pH, incubation time, and incubation temperature on the production of avicin A, as indicated by the diameter of the zone of inhibition.

Factor	Zone of inhibition diameter (mm)*
pH:
4	0
5	0
6	13.67 ± 0.33
7	19 ± 1a
8	21.33 ± 1.33b
Incubation time (hours):
6	12.33 ± 0.66
12	13.83 ± 0.44
18	18.67 ± 0.33c
24	15.33 ± 1.20
30	20.67 ± 1.20d
Incubation temperature (°C):
20	16.67 ± 1.20e
25	12 ± 0.57
30	18.67 ± 1.20f
37	13.83 ± 0.16
40	8.33 ± 0.88

*Data representing the average of triplicate values, are shown as mean ± standard error of the mean. ^aSignificantly different from pH values (4, 5, 6), p < 0.05. ^bSignificantly different from pH values (4, 5, 6), p < 0.05. ^cSignificantly different from incubation time values (6, 12 hours), p < 0.05. ^dSignificantly different from incubation time values (6, 12, 24 hours), p < 0.05. ^eSignificantly different from incubation temperature values (25, 40 °C), p < 0.05. ^fSignificantly different from incubation temperature values (25, 37, 40 °C), p < 0.05.